Andrey Poloznikov
- Associate Professor:Faculty of Biology and Biotechnology / Joint Department with RAS Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
- Leading Research Fellow:Faculty of Biology and Biotechnology / International Laboratory of Microphysiological Systems
- Andrey Poloznikov has been at HSE University since 2020.
Education and Degrees
- 2008
Candidate of Sciences* (PhD)
Lomonosov Moscow State University - 2005
Degree in Chemistry
Lomonosov Moscow State University
According to the International Standard Classification of Education (ISCED) 2011, Candidate of Sciences belongs to ISCED level 8 - "doctoral or equivalent", together with PhD, DPhil, D.Lit, D.Sc, LL.D, Doctorate or similar. Candidate of Sciences allows its holders to reach the level of the Associate Professor.
Courses (2021/2022)
- Research Methods of Biological Macromolecules (Bachelor’s programme; Faculty of Biology and Biotechnology; 3 year, 1, 2 module)Rus
- Past Courses
Courses (2020/2021)
Publications25
- Article Poloznikov A., Nikulin S., Bolotina L., Kachmazov A., Raigorodskaya M., Kudryavtseva A., Bakhtogarimov I., Rodin S., Gaisina I., Topchiy M., Asachenko A., Novosad V., Tonevitsky A., Alekseev B. 9-ING-41, a Small Molecule Inhibitor of GSK-3β, Potentiates the Effects of Chemotherapy on Colorectal Cancer Cells // Frontiers in Pharmacology. 2021. Vol. 12. Article 777114. doi
- Article Ahuja M., Ammal Kaidery N., Attucks O. C., McDade E., Hushpulian D. M., Gaisin A., Gaisina I., Ahn Y. H., Nikulin S., Poloznikov A., Gazaryan I. G., Yamamoto M., Matsumoto M., Igarashi K., Sharma S. M., Thomas B. Bach1 derepression is neuroprotective in a mouse model of Parkinson’s disease // Proceedings of the National Academy of Sciences of the United States of America. 2021. Vol. 118. No. 45. Article e2111643118. doi
- Article Hushpulian D. M., Kaidery N. A., Ahuja M., Poloznikov A., Sharma S. M., Thomas B., Gazaryan I. G. Challenges and Limitations of Targeting the Keap1-Nrf2 Pathway for Neurotherapeutics: Bach1 De-Repression to the Rescue // Frontiers in Aging Neuroscience. 2021. Vol. 13. P. 1-8. doi
- Article Nikulin S., Zakharova G., Poloznikov A., Raigorodskaya M., Wicklein D., Schumacher U., Nersisyan S., Bergquist J., Bakalkin G., Astakhova L., Tonevitsky A. Effect of the Expression of ELOVL5 and IGFBP6 Genes on the Metastatic Potential of Breast Cancer Cells // Frontiers in Genetics. 2021. Vol. 12. Article 662843. doi
- Article Poloznikov A., Nersisyan S., Hushpulian D., Kazakov E., Tonevitsky A., Kazakov S., Vechorko V., Nikulin S., Makarova J., Gazaryan I. G. HIF Prolyl Hydroxylase Inhibitors for COVID-19 Treatment: Pros and Cons // Frontiers in Pharmacology. 2021. Vol. 11. Article 621054. doi
- Article Leonardo C., Zumpano R., Poloznikov A., Hushpulian D. M., Tishkov V. I., Andreu R., Gorton L., Mazzei F., Favero G., Bollella P. Sensitive Hydrogen Peroxide Biosensor based on Tobacco Peroxidase Immobilized on p-Phenylenediamine Diazonium Grafted CNTs Preventing Fenton-like Inactivation at Negative Potential // ChemElectroChem. 2021. Vol. 8. No. 13. P. 2495-2504. doi
- Article Хушпульян Д. М., Никулин С. В., Христиченко А. Ю., Полозников А. А., Газарян И. Г., Чубарь Т. А. Гены «быстрого» отклика при действии ингибиторов HIF пролилгидроксилазы // Вестник Московского университета. Серия 2: Химия. 2021. Т. 62. № 3. С. 213-222. doi
- Article Nersisyan S., Shkurnikov M., Poloznikov A., Turchinovich A., Burwinkel B., Anisimov N., Tonevitsky A. A post-processing algorithm for miRNA microarray data // International Journal of Molecular Sciences. 2020. Vol. 21. No. 4. P. 1228. doi
- Article Nikulin S., Alekseev B. Y., Sergeeva N., Karalkin P., Nezhurina E., Kirsanova V., Sviridova I., Akhmedova S., Volchenko N., Bolotina L., Osipyants A., Hushpulian D., Topchiy M., Asachenko A., Koval A., Shcherbo D., Kiselev V., Mikhaylenko D., Schumacher U., Poloznikov A. Breast cancer organoid model allowed to reveal potentially beneficial combinations of 3,3′-diindolylmethane and chemotherapy drugs // Biochimie. 2020. Vol. 179. P. 217-227. doi
- Article Vlasova K., Vishwasrao H., Abakumov M., Golovin D., Gribanovsky S., Zhigachev A., Poloznikov A., Majouga A., Golovin Y., Sokolsky-Papkov M., Klyachko N., Kabanov A. Enzyme Release from Polyion Complex by Extremely Low Frequency Magnetic Field // Scientific Reports. 2020. Vol. 10. P. 4745. doi
- Article Olloqui-Sariego J. L., Zakharova G. S., Poloznikov A., Calvente J. J., Hushpulian D. M., Gorton L., Andreu R. Influence of tryptophan mutation on the direct electron transfer of immobilized tobacco peroxidase // Electrochimica Acta. 2020. Vol. 351. P. 136465. doi
- Article Savyuk M., Krivonosov M., Mishchenko T., Gazaryan I. G., Ivanchenko M., Khristichenko A., Poloznikov A., Hushpulian D., Nikulin S., Tonevitsky E., Abuzarova G., Mitroshina E., Vedunova M. Neuroprotective Effect of HIF Prolyl Hydroxylase Inhibition in an In Vitro Hypoxia Model // Antioxidants. 2020. Vol. 9. No. 8. Article 662. doi
- Article Poloznikov A., Nikulin S., Zakhariants A. A., Khristichenko A. Y., Hushpulian D. M., Gazizov I. N., Tishkov V. I., Gazaryan I. G. "Branched Tail" Oxyquinoline Inhibitors of HIF Prolyl Hydroxylase: Early Evaluation of Toxicity and Metabolism Using Liver-on-a-chip // Drug Metabolism Letters. 2019. Vol. 13. No. 1. P. 45-52. doi
- Article Yu.A. Makarova, Poloznikov A.A., Nikulin S.V., Raigorodskaya M., Fomicheva K. A., Zakharova G., Alekseev B. Y. Changes in the Metastatic Properties of MDA-MB-231 Cells after IGFBP6 Gene Knockdown Is Associated with Increased Expression of miRNA Genes Controlling INSR, IGF1R, and CCND1 Genes // Bulletin of Experimental Biology and Medicine. 2019. Vol. 166. No. 5. P. 641-645. doi
- Article Gerasimenko T., Nikulin S., Zakharova G., Poloznikov A., Petrov V., Baranova A., Tonevitsky A. Impedance Spectroscopy as a Tool for Monitoring Performance in 3D Models of Epithelial Tissues // Frontiers in Bioengineering and Biotechnology. 2019. Vol. 7. P. 474. doi
- Article Shkurnikov M., Nikulin S., Nersisyan S., Poloznikov A., Zaidi S., Baranova A., Schumacher U., Wicklein D., Tonevitsky A. LAMA4-Regulating miR-4274 and Its Host Gene SORCS2 Play a Role in IGFBP6-Dependent Effects on Phenotype of Basal-Like Breast Cancer // Frontiers in Molecular Biosciences. 2019. Vol. 6. No. 122. P. 1-7. doi
- Article Shkurnikov M., Nikulin S., Nersisyan S., Poloznikov A., Tonevitsky A. LAMA4-Regulating miR-4274 and Its Host Gene SORCS2 Play a Role in IGFBP6-Dependent Effects on Phenotype of Basal-Like Breast Cancer // Frontiers in Molecular Biosciences. 2019. Vol. 6. No. 8. P. 122. doi
- Article Olloqui-Sariego J., Zakharova G., Poloznikov A.A., Calvente J., Hushpulian D., Gorton L., Andreu R. The Fe (III)/Fe(II) redox couple as a probe of immobilized tobacco peroxidase: Effect of the immobilization protocol // Electrochimica Acta. 2019. Vol. 299. P. 55-61. doi
- Article Sakharov D., Maltseva D., Knyazev E., Nikulin S., Poloznikov A., Shilin S., Baranova A., Tsypina I., Tonevitsky A. Towards embedding Caco-2 model of gut interface in a microfluidic device to enable multi-organ models for systems biology // BMC Systems Biology. 2019. Vol. 13. No. 19. P. 3-11. doi
- Article Gaisina I. N., Lee S. H., Kaidery N. A., Aissa M. B., Ahuja M., Smirnova N. N., Wakade S., Gaisin A., Bourassa M. W., Ratan R. R., Nikulin S., Poloznikov A., Thomas B., Thatcher G. R., Gazaryan I. Activation of Nrf2 and Hypoxic Adaptive Response Contribute to Neuroprotection Elicited by Phenylhydroxamic Acid Selective HDAC6 Inhibitors // ACS Chemical Neuroscience. 2018. Vol. 9. No. 5. P. 894-900. doi
- Article Bollella P., Medici L., Tessema M., Poloznikov A., Hushpulian D., Tishkov V., Andreu R., Leech D., Megersa N., Marcaccio M., Gorton L., Antiochia R. Highly sensitive, stable and selective hydrogen peroxide amperometric biosensors based on peroxidases from different sources wired by Os-polymer: A comparative study. // Solid State Ionics. 2018. Vol. 314. P. 178-186. doi
- Article Poloznikov A., Gazaryan I. G., Shkurnikov M., Nikulin S., Drapkina O., Baranova A., Tonevitsky A. In vitro and in silico liver models: Current trends, challenges and opportunities // ALTEX-ALTERNATIVES TO ANIMAL EXPERIMENTATION. 2018. Vol. 35. No. 3. P. 397-412. doi
- Article Osipyants A., Smirnova N., Poloznikov A., Hushpulian D., Khristichenko A., Chubar T., Zakhariants A., Ahuja M., Gaisina I., Thomas B., Gazaryan I., Tishkov V. L-ascorbic acid: A true substrate for HIF prolyl hydroxylase? // Biochimie. 2018. Vol. 147. P. 46-54. doi
- Article Knyazev E., Poloznikov A., Maltseva D., Khristichenko A. Oxyquinoline derivative activates HIF-1 and increases transepithelial resistance of BeWo b30 monolayer // Placenta. 2018. Vol. 69. P. e47. doi
- Article Ivashchenko D. V., Rudik A. V., Poloznikov A., Nikulin S., Smirnov V. V., Tonevitsky A., Bryun E. A., Sychev D. A. Which cytochrome P450 metabolizes phenazepam? Step by step in silico, in vitro, and in vivo studies // Drug Metabolism and Personalized Therapy. 2018. Vol. 33. No. 2. P. 65-73. doi
Employment history
2017-2020 National Medical Research Radiological Center, Moscow, Russia
DEPUTY DIRECTOR FOR SCIENCE
Integration of state of the art methods to routine diagnostics: liquid biopsy using droplet PCR for melanoma, lung and thyroid cancers; small panel NGS genomic profiling for breast, ovarian and prostated cancers; comprehensive genomic profiling for lung and breast cancers (first study in Russia, 300 patients has been enrolled).
Development of drug test approach using ex vivo culturing of patient-derived three-dimensional tumor organoids and utilizing this model as a platform for preclinical studies of anticancer drugs and personalized treatment
2016-2018 Bioclinicum SRC, Moscow, Russia
PRINCIPAL SCIENTIST
Work on the development of new approaches for preclinical drug testing.
Developing organ-on-chip technology (liver, brain-blood barrier).
Organ-on-chip devices developing.
2011-2019 Federal Research and Clinical Centre of Pediatric Hematology, Oncology and Immunology, Moscow, Russia
LABORATORY HEAD
Drug monitoring.
Research on the leukemia cells chemotherapy resistance. Proteomics, metabolomics and epigenettics studies.
Hematology diseases diagnostics (porphyrias and Hemoglobinopathies). Neuroblastoma diagnostics.
Developing of novel drugs-inhibitors of Hypoxia inducible factor prolyl hydroxilases (HIF PHD).
2011-2016 Peroxo LLC, Moscow, Russia
PROJECT MANAGER
Optimizing technology of native/transgenic and recombinant tobacco peroxidase isolation, SAR research.
Optimizing technology of native and recombinant horseradish peroxidase isolation.
2008-2012 Biochemmack JSC, Moscow, Russia
PRODUCT MANAGER
Product manager and application specialist for the following product lines: microplate readers, automated ELISA analysers
Microarray genetic tests systems: CGH microarray assays for aneuploidy detection
2007-2008 Cornell University Burke Medical Research Institute, NY, USA
SHORT TERM SCHOLAR
Refolding optimization, isolation and characterization of MICAL – axon guidance protein
2007-2008 Lomonosov Moscow State University Moscow, Russia
JUNIOR SCIENTIST
Recombinant tobacco peroxidase: structure – properties relationship.
Isolation and characterization of recombinant plant peroxidases of different origins