Design of Antibacterial Peptides with Reduced Hemolytic Activity on the Basis of Linear Cytolytic Peptides from Spider Venom

Antimicrobial peptides are alternative to classical antibiotics. However, their use is hindered by their undesirable activity against erythrocytes, hemolytic activity. At the moment there are no programs for calculating the hemolytic activity of peptides in which only the amino acid sequence of the peptide is used. The task of this work is to develop an algorithm for predicting the hemolytic activity of peptides with a length of 20-40 amino acid residues based on their free energy of incorporation into the zwitterionic lipid bilayer. As a result, 2 equations based on the multiple regression model were obtained. The obtained equations can in most cases, in good agreement with the experimental data, predict the hemolytic activity of linear peptides. In addition, there is a literature review, which presents the current state of the problem of the relationship between the hemolytic activity of peptides and their biophysical properties.